Search

Photodynamic therapy on a biofilm monospecie of candida albicans: an in vitro study

Authors

Abstract

 

VOLUME 9 - NUMBER 2 - 2018 Photodynamic therapy on a biofilm monospecie of candida albicans: an in vitro study
  • Palaia G., Tenore G., Del Vecchio A., Pergolini D., Tramutola L., Berlutti F., Romeo U.
  • Original Article, 65-71
  • Full text PDF
  •  

Antimicrobial photodynamic therapy (aPDT) is a medical treatment based on the use of a light source at a specific wavelength that activates a photosensitive molecule causing the formation of oxidizing agents that provokes the death of the target cells. The aim of this study was to evaluate the activity of aPDT against biofilms of Candida albicans. Biofilms of C.albicans were subjected to aPDT using a diode GaAlAs (λ:635nm) and a toluidin blue solution (0.1mg/ml) as photosensitizer. Three different protocols (1 minute, fluence 24J/cm2; 2 minutes, fluence 48J/cm2 and 3 minutes, fluence 72J/cm2) were tested. To evaluate the number of Candida cells in biofilm before and after treatment, Bio Timer Assay was used. Candida biofilms treated with aPDT showed significant reductions of the microbial population as compared to the control groups. aPDT showed good antifungal properties in vitro. Clinical trials are needed to test its efficacy in vivo and to propose it as an adjuvant or alternative therapy to conventional medical treatment.

Share on

Downloads

Authors

G. Palaia

G. Tenore

A. Del Vecchio

D. Pergolini

L. Tramutola

F. Berlutti

U. Romeo

How to Cite
Palaia , G., Tenore , G., Del Vecchio, A., Pergolini , D., Tramutola , L., Berlutti , F., & Romeo , U. (2022). Photodynamic therapy on a biofilm monospecie of candida albicans: an in vitro study. Annali Di Stomatologia, 9(2), 65–71. https://doi.org/10.59987/ads/2018.2.65-71

Most read articles by the same author(s)

<< < 1 2